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Background on Reactive Oxygen Species (ROS) Testing

Reactive oxygen species (ROS) are chemically active oxygen-containing molecules, including H2O2 (hydrogen peroxide), NO (nitric oxide), O2- (oxide anion), peroxynitrite (ONOO-), hydrochloric acid (HOCl) , And hydroxyl radical (OH-). Low levels of ROS act as messengers in normal cell signal transduction, cell cycle, gene expression and homeostasis. When ROS accumulate in cells in large quantities, it is also called oxidative stress. They can damage DNA, RNA, proteins, and even cause cell death. Reactive oxygen biomolecules are involved in a series of pathological processes, including cancer, neurodegenerative diseases, atherosclerosis and aging processes. Since reactive oxygen species affect many biological activities, they have been extensively studied. Measuring ROS is used to report immune responses, monitor the balance of reactive oxygen species and antioxidants, or study their role in cancer development. However, detecting active oxygen levels can be difficult because they are usually in the nanomolar range and have a short half-life.

Reactive Oxygen Species (ROS) Test Kits

Reactive oxygen species are measured in cancer research, immunology or food laboratories. The Reactive Oxygen Detection Kit is the most commonly used method for quantitative detection of intracellular reactive oxygen species based on changes in the fluorescence intensity of the fluorescent dye DCFH-DA (2,7-Dichlorodi-hydrofluorescein diacetate).

DCFH-DA can freely penetrate the living cell membrane into the cell, and is hydrolyzed by intracellular esterase to form DCFH. DCFH is non-fluorescent and cannot penetrate the cell membrane. It can be oxidized by intracellular ROS to generate fluorescent DCF. Use flow cytometer or fluorescence microscope to detect or observe the fluorescence signal intensity of DCF in living cells, which can analyze the level of cellular reactive oxygen species.

Optimized incubation regime for nitric oxide measurements in murine macrophages using the Griess assay. Fig 1. Methods for the detection of reactive oxygen species. (Zhang Y, et al, 2018)

Advantages of Reactive Oxygen Species (ROS) Test Kits

  • Use the ultra-sensitive fluorescent probe DCFH-DA to detect reactive oxygen species.
  • The kit contains instructions and provides detailed operating steps.
  • It is suitable for direct observation by laser confocal microscope, fluorescence spectrophotometer, fluorescence microplate reader, flow cytometer and fluorescence microscope to detect the level of reactive oxygen species.
  • The kit has low cost, high sensitivity, wide linear range and convenient use.
  • It can measure 100 to 500 samples.

CD BioSciences provides kits for detecting reactive oxygen species using the fluorescent probe DCFH-DA. In addition, our scientists are focusing on developing a probe that can selectively detect superoxide produced in mitochondria during oxidative phosphorylation. We are committed to producing fast, efficient, safe and inexpensive reactive oxygen species (ROS) test kits to meet your requirements. If you are interested in our products, welcome to contact us for products quotations and more detailed information.

Reference

  • 1. Zhang Y, Dai M, Yuan Z. (2018) Methods for the detection of reactive oxygen species. Analytical Methods. 10(38): 4625-4638.
  • It should be noted that our products are for research purposes only. Not suitable for any clinical use.

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